Abstrak Phylogenetic Evidence of Cryptic Species in Giant Fetaherback (Chitala lopis Bleeker 1851)

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Authors

,Arif Wibowo,Dwi Atminarso,

The successes of biology conservation effort rely on the ability to identify species level and determine of the population unit. Molecular marker can assist identification population unit precisely. Molecular technique revealed hidden diversity on species with similar morphology performed. The typical diverse specific habitat of giant featherback (reservoir, floodplain, tributary and river) and geography separation of the species have been contributed to the existed of the cryptic species. Research objective is to reveal the evidence cryptic species of giant featherback, in order to make contribution either for in-situ and ex-situ conservation and adding more information on giant featherback‘s biodiversity for breeding purpose. Sequence data were obtained from Cytochrome Oxidase Subunit I (COI) and Cytochrome b mtDNA. Samples total are 28 individuals collected from five different river. We compare 5 samples of Chitala lopis and Notophterus notopterus sequences from Genebank as standard samples. Results reveal molecular filogeny analysis from COI and Cyt b nucleotide of mtDNA indicated that Indonesia Chitala lopis can be distinguised minimal four clusters as cryptic species, which were Chitala lopis, Borneo,  giant featherback (totol) dan giant featherback in acid environment. The evidence cryptic species added the importance of molecular analysis techniques beside morphological whilst cataloge biodiversity and determine conservation prioroties.

Keywords: Chitala lopis, mtDNA and cryptic species