Abstrak The Genetic Structure of The White Cyprind (Tor tambroides) Population Based on COI Gene Sequance Analyzes

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Authors

,Arif Wibowo,Husnah,

The  study analyzed  nucleotide  sequences from  the  mitochondrial  cytochrome  oxidase  subunit COi)  gene  region  (654) to investigate  the  genetic structure  of the  white  cyprinid  (Tor tambroides) among  nine populations from the Manna and Semanka  Rivers. A total of 36 individuals were collected fur this  work.  Five  nucleotides were  found to  be  variable,  resulting in  4 haplotypes.  Among  the nine pc. oulations of Melebuy in  Semanka  River represents  the highest level of variability (h  =  1.000,  Ò=0.0015) whereas  Kerinjing,  Merabung  and  Kotabumi populations  represent  the  highest  level of ,-a'lability in Manna River (h = 0.667, Ò= 0.0020).  The Batu Aji, Air Sebilo and Kutopadang populations exr, bit the lowest level of variability (h = 0.000, Ò= 0.000).  There is an integrated population throughout sample  sites  in  Manna  River.  However.  the AMOVA  analysis provided corroborating  evidence for ge· etic structure obtained  from  Nei's genetic  diversity statistic  and the  F_ST  value  (0.310),  suggested ere 1s genetic divergence among populations of those populations.  Of the total genetic diversity.  35% was  attributable to inter-population  diversity  and  the  remainder  (68.92%)  to differences  within populations.  These  two approaches produced a picture  of genetic  structure in  Manna  and  Semanka River.  A molecular phylogenetic tree constructed using  The Neighbor-joining (NJ) method showed the 4 haplotypes  were assigned to two clades associated geographic regions.  These results provide  basic information  for the conservation  and sustainable  exploitation  of this  species.

Keywords: Tor tambroides, COI genes, genetic struture, genetic variation, haplotype